
To determine the polymorphism of mutT genes of Mycobacterium tuberculosis of Beijing genotype, we developed a duplex real-time PCR assay based on hybridization probes for the Roche LightCycler instrument. The assay rapidly detects mutations at codons 48 and 58 of genes mutT4 and at mutT2, respectively.
Bacteriological Techniques, Polymorphism, Genetic, Bacterial Proteins, Genotype, Mutation, Missense, Humans, Mycobacterium tuberculosis, Polymerase Chain Reaction
Bacteriological Techniques, Polymorphism, Genetic, Bacterial Proteins, Genotype, Mutation, Missense, Humans, Mycobacterium tuberculosis, Polymerase Chain Reaction
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