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Nucleic Acids Symposium Series
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Nucleic Acids Symposium Series
Article . 2009 . Peer-reviewed
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Nucleic Acids Symposium Series
Article . 2010
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Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Sep 2009 English Publisher:Oxford University Press (OUP)Journal:Nucleic Acids Symposium Series, volume 53, pages 295-296 (issn: 0261-3166, eissn: 1746-8272, Copyright policy )
Authors: Takashi Yokogawa; Hanim Munirah Binti Che Hassan; Satoshi Ohno; Yukiko Yokota; Kazuya Nishikawa;

doi: 10.1093/nass/nrp148

pmid: 19749377

Preparation of an ochre suppressor tRNA recognizing exclusively UAA codon by using the molecular surgery technique

Abstract

In order to create an ochre suppressor tRNA which exclusively recognizes UAA codon, we replaced the G34 at the first position of yeast tRNA(Tyr)[GPsiA] anticodon with pseudouridine34 (Psi34) by using the molecular surgery technique. This tRNA(Tyr)[PsiPsiA] recognized only the UAA codon as expectedly, but tRNA(Tyr)[UPsiA] made as a control also behaved similarly. This result may suggest that U34 must be somehow modified to facilitate the wobble-pairing to G at the third position of codon.

Related Organizations
Keywords

RNA, Transfer, Tyr, Genetic Techniques, Anticodon, Codon, Terminator, Pseudouridine

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
bronze