Separation of Dengue Strains on the Basis of a Nonstructural Antigen
Copyright policy )Separation of Dengue Strains on the Basis of a Nonstructural Antigen
Abstract Intratypic variation of the nonstructural soluble complement-fixing (SCF) antigens of dengue-1 strains (Hawaii and TH-Sman) and dengue-2 strains (New Guinea C and TH-36) were examined by immunological and biophysical techniques. The SCF antigen of Hawaii could be separated from that of TH-Sman by subtle differences in CF cross reactions but by distinct differences in their relative mobilities when tested by disc-gel electrophoresis. SCF antigens of dengue-2 strains could not be distinguished by any of the methods used. We suggest that the SCF antigen is responsible for the strain variation observed in the two dengue-1 strains.
Immunodiffusion, Virus Cultivation, Complement Fixation Tests, Cross Reactions, Dengue Virus, Electrophoresis, Disc, Antibodies, Mice, Methods, Animals, Female, Antigens, Antigens, Viral
Immunodiffusion, Virus Cultivation, Complement Fixation Tests, Cross Reactions, Dengue Virus, Electrophoresis, Disc, Antibodies, Mice, Methods, Animals, Female, Antigens, Antigens, Viral
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