Specific Detection of Bacillus Anthracis Using Ataqman® Mismatch Amplification Mutation Assay
Copyright policy )Specific Detection of Bacillus Anthracis Using Ataqman® Mismatch Amplification Mutation Assay
Single nucleotide polymorphisms (SNPs) are increasingly recognized as important diagnostic markers for the detection and differentiation of Bacillus anthracis. The use of SNP markers for identifying B. anthracis DNA in environmental samples containing genetically similar bacteria requires the ability to amplify and detect DNA with single nucleotide specificity. We designed a TaqMan mismatch amplification mutation assay (TaqMAMA) around a SNP in the plcR gene of B. anthracis. The assay permits specific, low-level detection (25 fg DNA) of this B. anthracis-specific SNP, even in the presence of environmental DNA extracts containing a 20,000-fold excess of the alternate allele. We anticipate that the ability to selectively amplify and detect low copy number DNAs with single nucleotide specificity will represent a valuable tool in the arena of biodefense and microbial forensics.
- Northern Arizona University United States
- Translational Genomics Research Institute United States
QH301-705.5, Base Pair Mismatch, DNA Mutational Analysis, Reproducibility of Results, Sequence Analysis, DNA, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sensitivity and Specificity, Bacillus anthracis, Taq Polymerase, Biology (General), Sequence Alignment, In Situ Hybridization
QH301-705.5, Base Pair Mismatch, DNA Mutational Analysis, Reproducibility of Results, Sequence Analysis, DNA, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sensitivity and Specificity, Bacillus anthracis, Taq Polymerase, Biology (General), Sequence Alignment, In Situ Hybridization
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