<script type="text/javascript">
<!--
document.write('<div id="oa_widget"></div>');
document.write('<script type="text/javascript" src="https://www.openaire.eu/index.php?option=com_openaire&view=widget&format=raw&projectId=undefined&type=result"></script>');
-->
</script>

COPY SCRIPT

For further information contact us at helpdesk@openaire.eu

Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 2017 English Publisher:Elsevier BVJournal:Neuroscience Letters, volume 653, pages 132-138 (issn: 0304-3940,

Authors: Ren-Shan Ge; Lili Li; Xiaoheng Li; Jingjing Guo; Songyi Zhou; Jianliang Sun; Ying Su;

doi: 10.1016/j.neulet.2017.05.034

pmid: 28552457

Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis

- Summary
- Subjects
- Related research
  (5)
- Metrics

Abstract

Butylated hydroxyanisole is a synthetic antioxidant. It may affect the function of the nerve system. The objective of the present study is to investigate the direct effects of butylated hydroxyanisole on rat brain neurosteroidogenic 5α-reductase 1 (SRD5A1), 3α-hydroxysteroid dehydrogenase (AKR1C14), and retinol dehydrogenase 2 (RDH2). Rat SRD5A1, AKR1C14, and RDH2 were cloned and expressed in COS1 cells, and the effects of butylated hydroxyanisole on these enzyme activities were measured. Butylated hydroxyanisole inhibited SRD5A1, AKR1C14, and RDH2 with IC50 values of 4.731±0.079μM, 5.753±0.073μM, and over 100μM, respectively. Butylated hydroxyanisole is a competitive inhibitor for both SRD5A1 and AKR1C14. Docking analysis shows that butylated hydroxyanisole binds to the dihydrotestosterone-binding site of AKR1C14. In conclusion, butylated hydroxyanisole is a potent inhibitor of SRD5A1 and AKR1C14, thus reducing the formation of active neurosteroids.

Related Organizations

Hangzhou First People's Hospital
China (People's Republic of)
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
China (People's Republic of)
Capital Medical University
China (People's Republic of)
Wenzhou Medical University
China (People's Republic of)
Nanjing Medical University
China (People's Republic of)

Keywords

Binding Sites, Butylated Hydroxyanisole, Membrane Proteins, Molecular Docking Simulation, Alcohol Oxidoreductases, 3-Oxo-5-alpha-Steroid 4-Dehydrogenase, COS Cells, Chlorocebus aethiops, Animals, Oxidoreductases

5 Research products, page 1 of 1

Placental neurosteroids shape cerebellar development and social behaviour
2019IsAmongTopNSimilarDocuments
Methoxychlor and its metabolite HPTE inhibit rat neurosteroidogenic 3α-hydroxysteroid dehydrogenase and retinol dehydrogenase 2
2018IsAmongTopNSimilarDocuments
Ziram inhibits rat neurosteroidogenic 5α-reductase 1 and 3α-hydroxysteroid dehydrogenase
2017IsAmongTopNSimilarDocuments
Stem Cell-Based Selective Delivery of Alpha Keto Reductases for Therapeutic Targeting of Residual Androgens in Metastatic Prostate Cancer
2016IsAmongTopNSimilarDocuments
Effects of Etomidate on the Steroidogenesis of Rat Immature Leydig Cells
2015IsAmongTopNSimilarDocuments

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	10
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average