An assessment of histone-modification antibody quality
Copyright policy )An assessment of histone-modification antibody quality
We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).
- Lawrence Berkeley National Laboratory United States
- University of California, Santa Cruz United States
- University of North Carolina System United States
- Ludwig Cancer Research United States
- University of North Carolina at Chapel Hill United States
Quality Control, Chromatin Immunoprecipitation, Blotting, Western, Immunoblotting, Biophysics, Medical and Health Sciences, Article, Antibodies, Histones, Antibody Specificity, Genetics, Animals, Drosophila Proteins, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Biology, Protein Processing, Blotting, Post-Translational, Reproducibility of Results, Biological Sciences, Drosophila melanogaster, Chemical Sciences, Generic health relevance, Western, Protein Processing, Post-Translational, Biotechnology, Developmental Biology
Quality Control, Chromatin Immunoprecipitation, Blotting, Western, Immunoblotting, Biophysics, Medical and Health Sciences, Article, Antibodies, Histones, Antibody Specificity, Genetics, Animals, Drosophila Proteins, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Biology, Protein Processing, Blotting, Post-Translational, Reproducibility of Results, Biological Sciences, Drosophila melanogaster, Chemical Sciences, Generic health relevance, Western, Protein Processing, Post-Translational, Biotechnology, Developmental Biology
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