The Aquatic Ecology Laboratory (Laboratorio de Ecología Acuática, LEA) of the University of Cuenca was formed in 2010 with the aim of generating knowledge on the biology and ecology of high mountain freshwater ecosystems (lotic and lentic). LEA carries out research projects within several research lines such as: freshwater biomonitoring, estimation of environmental flows, taxonomy of freshwater insects, algae, ecology of aquatic communities, use of remote sensing for water resource management, lake limnology, etc. The biological data obtained from both lakes and rivers are consolidated in the database. The dataset published contains: 747 records derived from monitoring of lakes and rivers in the Ecuador.