Structure of replicating SARS-CoV-2 polymerase
Copyright policy )Structure of replicating SARS-CoV-2 polymerase
The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) for the replication of its genome and the transcription of its genes. Here we present the cryo-electron microscopic structure of the SARS-CoV-2 RdRp in its replicating form. The structure comprises the viral proteins nsp12, nsp8, and nsp7, and over two turns of RNA template-product duplex. The active site cleft of nsp12 binds the first turn of RNA and mediates RdRp activity with conserved residues. Two copies of nsp8 bind to opposite sides of the cleft and position the RNA duplex as it exits. Long helical extensions in nsp8 protrude along exiting RNA, forming positively charged ‘sliding poles’ that may enable processive replication of the long coronavirus genome. Our results will allow for a detailed analysis of the inhibitory mechanisms used by antivirals such as remdesivir, which is currently in clinical trials for the treatment of coronavirus disease 2019 (COVID-19).
Models, Molecular, Multidisciplinary, Alanine, Coronavirus RNA-Dependent RNA Polymerase, Protein Conformation, SARS-CoV-2, Cryoelectron Microscopy, COVID-19, Viral Nonstructural Proteins, RNA-Dependent RNA Polymerase, Adenosine Monophosphate, Coronavirus, Betacoronavirus, Síndrome respiratorio agudo grave, Polimerasa, RNA, Viral, Polymerase
Models, Molecular, Multidisciplinary, Alanine, Coronavirus RNA-Dependent RNA Polymerase, Protein Conformation, SARS-CoV-2, Cryoelectron Microscopy, COVID-19, Viral Nonstructural Proteins, RNA-Dependent RNA Polymerase, Adenosine Monophosphate, Coronavirus, Betacoronavirus, Síndrome respiratorio agudo grave, Polimerasa, RNA, Viral, Polymerase
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).719 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 0.1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.01%
Citations provided by BIP!Popularity provided by BIP!