Changes in fatty acid and amino acid composition in hot smoked sturgeon (Huso huso, L. 1758) were studied. The sturgeon was smoked for 20 min at 30 degrees C, followed by 90 min at 50 degrees C and 40 min at 80 degrees C. The palmitic acid (C16:0) and stearic acid (C18:0) increased (P0.05) was observed in the total monounsaturated fatty acid composition of fish after smoking. Total polyunsaturated fatty acid, eicosapentaenoic acid and docosahexaenoic acid contents decreased from 21.03% to 4.93%, from 4.65% to 0.49% and from 12.41% to 0.51% in sturgeon after smoking, respectively. The total n-3 and n-6 contents and the n-3/n-6 ratio of raw and smoked sturgeon were 17.48-1.35, 3.54-3.58 and 4.94-0.38, respectively. The atherogenic index and index of thrombogenicity values of raw and smoked sturgeon were determined as 1.01-0.31 and 1.93-1.24, respectively. The smoking process caused an increase (Pisoleucine >methionine >hidoksil-1-proline >valine, and a decrease (Pserine >threonine >leucine >tyrosine >histidine >lysine >proline. However, the changes in alanine, glycine and phenylalanine were insignificant (P>0.05).