Tryptic cleavage of IgD at elevated temperature and isolation of a Fc-like fragment in high yield
Copyright policy )Tryptic cleavage of IgD at elevated temperature and isolation of a Fc-like fragment in high yield
Abstract Digestion of IgD with trypsin at 56°C for 4 min has been shown to be an effective method for the production of homogeneous Fc-like component in high yield. The first two residues of this fragment were found to be Thr-Ala. Because of rapid and continuous degradation this method does not allow production of Fab-like components in significant quantities.
- United States Department of Veterans Affairs United States
- Alabama Agricultural and Mechanical University United States
- University of Alabama at Birmingham United States
- Larue Carter Hospital United States
Hot Temperature, Immunoglobulin delta-Chains, Hydrolysis, Immunoglobulin D, Immunoglobulin Fc Fragments, Molecular Weight, Immunoglobulin lambda-Chains, Chromatography, Gel, Humans, Electrophoresis, Polyacrylamide Gel, Trypsin, Immunoelectrophoresis, Ultracentrifugation
Hot Temperature, Immunoglobulin delta-Chains, Hydrolysis, Immunoglobulin D, Immunoglobulin Fc Fragments, Molecular Weight, Immunoglobulin lambda-Chains, Chromatography, Gel, Humans, Electrophoresis, Polyacrylamide Gel, Trypsin, Immunoelectrophoresis, Ultracentrifugation
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