AbstractBio‐mimicked GTR/GBR membranes with hierarchical structured surfaces were developed by direct and indirect replication of teak leaf surface. The membranes were fabricated using solvent casting method with customized templates. The surfaces obtained were those with micro‐trichomes (MTS) and micro‐depression (MDS) that resembled a whorling pattern. Structural details of the fabricated membrane surfaces were studied under stereomicroscope and scanning electron microscopy. Surface roughness, water wetting angle, water uptake, and degradation properties of the membranes were examined. The effects of the micro‐patterned hierarchical structure on in vitro bioactivities of human osteoblast‐like cells (MG63) and human gingival fibroblast cells HGF1‐RT1 were studied. In vivo study carried out on rat skulls to assess the response of surrounding tissues for 4 weeks showed that the bio‐mimicked MTS and MDS membrane surfaces enhanced the cell proliferation. The proliferation significantly increased with increasing surface roughness and decreasing contact angle. There was also an evidence of rapid new bone maturation with membranes with MTS. It is thus suggested that the teak leaf mimicked whorling patterned hierarchical structured surface is an important design for enhancing bioactivity.