Additional file 1. Cytotoxicity assessment in human HepaRG™ cells following exposure to: (A) DDI chemicals in μM concentrations; and (B) non-DDI chemicals in mM concentrations (except COL, which was in μM) using the In Vitro MicroFlow® assay (Litron Laboratories). A low, mid and high concentration were used for AmpliSeq analysis (specific concentrations are underlined in Table 1). Percent relative survival is depicted 96 hr following the last exposure (n = 2). DDI chemical abbreviations: 2-nitrofluorene (2NF), cisplatin (CISP), etoposide (ETP), aflatoxin B1 (AFB1), and methyl methanesulfonate (MMS). Non-DDI chemical abbreviations: 2-deoxy-D-glucose (2DG), sodium chloride (NaCl), ampicillin trihydrate (AMP), sodium ascorbate (ASC), and colchicine (COL). Control represents the vehicle control (DMSO for 2NF, CISP, ETP, AFB1, and COL; water for MMS; media for 2DG, NaCl, AMP, and ASC). Error bars depict standard error, but are too small to visualize. * P < 0.05 compared to the vehicle control.