TOPLESS Mediates Auxin-Dependent Transcriptional Repression During Arabidopsis Embryogenesis
Copyright policy )TOPLESS Mediates Auxin-Dependent Transcriptional Repression During Arabidopsis Embryogenesis
The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR (ARF) proteins, which can activate transcription of auxin response genes. We show that TOPLESS (TPL) can physically interact with IAA12/BODENLOS (IAA12/BDL) through an ETHYLENE RESPONSE FACTOR (ERF)–associated amphiphilic repression (EAR) motif. TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity. In addition, tpl-1 can suppress the patterning defects of the bdl-1 mutant. Direct interaction between TPL and ARF5/MONOPTEROS, which is regulated by IAA12/BDL, results in a loss-of-function arf5 / mp phenotype. These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development.
- University of California, San Diego United States
- University of California, San Diego United States
- Salk Institute for Biological Studies United States
Indoleacetic Acids, Models, Genetic, Transcription, Genetic, Arabidopsis Proteins, Recombinant Fusion Proteins, Amino Acid Motifs, Arabidopsis, Protein Structure, Tertiary, DNA-Binding Proteins, Repressor Proteins, Gene Expression Regulation, Plant, Seedlings, Two-Hybrid System Techniques, Mutation, Seeds, Protein Binding, Transcription Factors
Indoleacetic Acids, Models, Genetic, Transcription, Genetic, Arabidopsis Proteins, Recombinant Fusion Proteins, Amino Acid Motifs, Arabidopsis, Protein Structure, Tertiary, DNA-Binding Proteins, Repressor Proteins, Gene Expression Regulation, Plant, Seedlings, Two-Hybrid System Techniques, Mutation, Seeds, Protein Binding, Transcription Factors
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