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pmid: 22503829
The success of Sazanov's group in determining the X-ray structure of the whole bacterial complex I is a great contribution to the progress of complex I research. In this mini-review of 35years' history of my laboratory and collaborators, we characterized the function of protein-associated semiquinone molecules in the proton-pumping mechanism in complex I (NADH-quinone oxidoreductase). We have constructed most of the frame work of our hypothesis, utilizing EPR techniques before the X-ray structures of complex I were reported by Sazanov's and Brandt's groups. One of the semiquinones (SQ(Nf)) is extremely sensitive to a proton motive force imposed on the energy-transducing membrane, while the other (SQ(Ns)) is insensitive. Their sensitivity to rotenone inhibition also differs. These differences were exploited using tightly coupled bovine heart submitochondrial particles with a high respiratory control ratio (>8). We determined the distance between SQ(Nf) and iron-sulfur cluster N2 on the basis of their direct spin-spin interaction. We are extending this line of work using reconstituted bovine heart complex I proteoliposomes which shows a respiratory control ratio >5. Two frontier research groups support our view point based on their mutagenesis studies. High frequency (33.9GHz; Q-band) EPR experiments appear to favor our two-semiquinone model. This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012).
Models, Molecular, Electron Transport Complex I, Biophysics, Electron Spin Resonance Spectroscopy, Muscle Proteins, Proton-Motive Force, Cell Biology, Crystallography, X-Ray, Biochemistry, SQ-Nf and SQ-Ns, Mitochondria, Heart, Proteoliposome, Mitochondrial Proteins, Oxygen Consumption, Proton-pumping mechanism, Benzoquinones, Animals, Cattle, EPR, Bovine heart complex I, Membrane in situ
Models, Molecular, Electron Transport Complex I, Biophysics, Electron Spin Resonance Spectroscopy, Muscle Proteins, Proton-Motive Force, Cell Biology, Crystallography, X-Ray, Biochemistry, SQ-Nf and SQ-Ns, Mitochondria, Heart, Proteoliposome, Mitochondrial Proteins, Oxygen Consumption, Proton-pumping mechanism, Benzoquinones, Animals, Cattle, EPR, Bovine heart complex I, Membrane in situ
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 26 | |
popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Top 10% | |
influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Top 10% |