Additional file 1: Table S1. Statistical analysis of stress resistance data. Accompanies Fig. 1. Worms of the strains indicated were subjected to heat or oxidative stress as specified in the individual table headlines. For each biological replicate of the respective experiment, the table lists mean and median survival times in h, standard deviations and standard errors of the mean, the number of worms scored, and p-values from Kaplan-Meier survival analysis with Mantel-Cox tests against the control-, daf-16(���) or mbk-1(���) strain of the same genetic background. In case of the GSC(���) and daf-2(���) control strain, survival analysis was performed relative to the GSC(+) or daf-2(+) control strain. Repeated measures two-way ANOVA with Bonferroni post tests was performed on the mean survival times determined in the individual biological replicates of the experiments. Statistically significant p-values (* p 1.5 (Log2FC > 0.58) for genes upregulated upon mbk-1 loss. Expression of these genes is normally repressed by mbk-1 (mbk-1 repressed DEGs). In addition, the table lists DEGs detected in control comparisons between the following strains (all mbk-1(+)): downregulated 1.5-fold in daf-2(���) relative to daf-2(+); downregulated 4-fold (Log2FC > 2) in GSC(���) relative to GSC(+). See Additional file 2 for full DESeq2-data. Table S4. Overlaps of mbk-1 dependent DEGs in the genetic backgrounds analyzed in this study. Accompanies Fig. 2c, d. (a) Pairwise overlaps between DEG-lists. For each overlap, the number of genes shared between the two particular lists, the representation factor, and the p-value (in italics; hypergeometric probability) are indicated. Statistically significant overlaps are highlighted in blue. (b) Absolute numbers of mbk-1 dependent DEGs that are induced/repressed exclusively in the background indicated, or that are also induced/repressed in one or more of the three other backgrounds. (c) Percentages of mbk-1 dependent DEGs that are induced/repressed exclusively in the background indicated, or that are also induced/repressed in one or more of the three other backgrounds. Table S5. Overlap of genes regulated by mbk-1 in this work and by daf-16 in published studies. For each overlap, the number of genes shared between the two particular lists, the representation factor, and the p-value (in italics; hypergeometric probability) are indicated. Statistically significant overlaps (p