Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum : two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene
Copyright policy )Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum : two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene
The DNA sequences of cytochrome oxidase (subunits 1, 2 and 3) genes of the cellular slime mold Dictyostelium discoideum mitochondria were determined. The genes for subunits 1 and 2 have a single continuous ORF (COX1/2) which contains four group-I introns. The insertion sites of the two group-I introns (DdOX1/2.2 and DdOX1/2.3) coincide with those of fungal and algal group-I introns, as well as a liverwort group-I intron, in the cytochrome oxidase subunit 1. Interestingly, intron DdOX1/2.2 has two free-standing ORFs in a loop (L8) which have similar amino-acid sequences and are homologous to ai4 DNA endonuclease (I-Sce II) and bi4 RNA maturase found in group-I introns of Saccharomyces cerevisiae mitochondrial DNA. Two group-I introns (DdOX1/2.3 and DdOX1/2.4) also have a free-standing ORF in loop 1 and loop 2, respectively. These results show that these group-I introns and the intronic ORFs have evolved from the same ancestral origin, but that these ORFs have been propagated independently.
- University of Tsukuba Japan
Saccharomyces cerevisiae Proteins, Molecular Structure, Molecular Sequence Data, Chromosome Mapping, Membrane Proteins, Saccharomyces cerevisiae, Endonucleases, DNA, Mitochondrial, Nucleotidyltransferases, Introns, DNA-Binding Proteins, Electron Transport Complex IV, Open Reading Frames, Endoribonucleases, Animals, Dictyostelium, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Phylogeny
Saccharomyces cerevisiae Proteins, Molecular Structure, Molecular Sequence Data, Chromosome Mapping, Membrane Proteins, Saccharomyces cerevisiae, Endonucleases, DNA, Mitochondrial, Nucleotidyltransferases, Introns, DNA-Binding Proteins, Electron Transport Complex IV, Open Reading Frames, Endoribonucleases, Animals, Dictyostelium, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Phylogeny
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