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Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

Research datakeyboard_double_arrow_right Dataset 10 Sep 2019Publisher:Zenodo

Authors: Mann, Mandeep; Harding, Rachel; Schapira, Matthieu;

doi: 10.5281/zenodo.3403803 , 10.5281/zenodo.3403804

Growth and Purification of Biotinylated USP5 Constructs & Testing ZnF-UBD Ligands in a Displacement Assay

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Abstract

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Expression and purification of biotinylated full length (FL) USP51-835 and biotinylated zinc finger ubiquitin-binding domain (ZnF-UBD) USP5171-290 constructs for the development of a displacement assay.

Related Organizations

University of Toronto
Canada

Keywords

zinc finger, usp5, displacement assay, protein purification

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