DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus
Copyright policy )DNA methylation changes in cell line from β-lactoglobulin gene targeted fetus
The gene targeting combined somatic cell nuclear transfer is very useful in agriculture and medicine. Epigenetic modification of DNA by methylation is significant in regulating gene expression during mammalian development. During gene targeting, epigenetic status of donor cell nuclei may be changed in a series of processes, including homologous recombination, cell selection and cloning. We examined DNA methylation of six genes (beta-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence art2 in blg(+/-) cell line from beta-lactoglobulin (BLG) gene targeted fetus and the cells used for BLG gene targeting serve as control. The results demonstrated that the widespread changes of DNA methylation were found in blg(+/-) cell line. But the degree of variation was different. DNA methylation of VEGF in blg(+/-) was noticeably decreased. These observations suggest that DNA methylation variations may impact gene expression and finally induce abnormalities and lethality in later developmental stages.
- China Agricultural University China (People's Republic of)
- Shenyang Pharmaceutical University China (People's Republic of)
ADP Ribose Transferases, Vascular Endothelial Growth Factor A, RNA, Untranslated, Gene Expression Regulation, Developmental, Lactoglobulins, DNA Methylation, Cell Line, Animals, Genetically Modified, Fetus, Insulin-Like Growth Factor II, Gene Targeting, Animals, Cattle, CpG Islands, RNA, Long Noncoding, Octamer Transcription Factor-3, Telomerase
ADP Ribose Transferases, Vascular Endothelial Growth Factor A, RNA, Untranslated, Gene Expression Regulation, Developmental, Lactoglobulins, DNA Methylation, Cell Line, Animals, Genetically Modified, Fetus, Insulin-Like Growth Factor II, Gene Targeting, Animals, Cattle, CpG Islands, RNA, Long Noncoding, Octamer Transcription Factor-3, Telomerase
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).5 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!