The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern
Copyright policy )The mouse lpA3/Edg7 lysophosphatidic acid receptor gene: genomic structure, chromosomal localization, and expression pattern
The extracellular signaling molecule, lysophosphatidic acid (LPA), mediates proliferative and morphological effects on cells and has been proposed to be involved in several biological processes including neuronal development, wound healing, and cancer progression. Three mammalian G protein-coupled receptors, encoded by genes designated lp (lysophospholipid) receptor or edg (endothelial differentiation gene), mediate the effects of LPA, activating similar (e.g. Ca(2+) release) as well as distinct (neurite retraction) responses. To understand the evolution and function of LPA receptor genes, we characterized lp(A3)/Edg7 in mouse and human and compared the expression pattern with the other two known LPA receptor genes (lp(A1)/Edg2 and lp(A2)/Edg4non-mutant). We found mouse and human lp(A3) to have nearly identical three-exon genomic structures, with introns upstream of the coding region for transmembrane domain (TMD) I and within the coding region for TMD VI. This structure is similar to lp(A1) and lp(A2), indicating a common ancestral gene with two introns. We localized mouse lp(A3) to distal Chromosome 3 near the varitint waddler (Va) gene, in a region syntenic with the human lp(A3) chromosomal location (1p22.3-31.1). We found highest expression levels of each of the three LPA receptor genes in adult mouse testes, relatively high expression levels of lp(A2) and lp(A3) in kidney, and moderate expression of lp(A2) and lp(A3) in lung. All lp(A) transcripts were expressed during brain development, with lp(A1) and lp(A2) transcripts expressed during the embryonic neurogenic period, and lp(A3) transcript during the early postnatal period. Our results indicate both overlapping as well as distinct functions of lp(A1), lp(A2), and lp(A3).
- University of California, San Diego United States
- University of California, San Diego United States
Male, DNA, Complementary, Base Sequence, Molecular Sequence Data, Mice, Inbred Strains, Receptors, Cell Surface, DNA, Blotting, Northern, Receptors, G-Protein-Coupled, Mice, Inbred C57BL, Alternative Splicing, Mice, Chromosomes, Human, Pair 1, Animals, Humans, Female, Amino Acid Sequence, RNA, Messenger, Receptors, Lysophosphatidic Acid, Sequence Alignment
Male, DNA, Complementary, Base Sequence, Molecular Sequence Data, Mice, Inbred Strains, Receptors, Cell Surface, DNA, Blotting, Northern, Receptors, G-Protein-Coupled, Mice, Inbred C57BL, Alternative Splicing, Mice, Chromosomes, Human, Pair 1, Animals, Humans, Female, Amino Acid Sequence, RNA, Messenger, Receptors, Lysophosphatidic Acid, Sequence Alignment
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