Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway
Copyright policy )Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway
We report a novel protein family consisting of three members, each of which contains RUN and TBC motifs and appears to be associated with small G protein-mediated signal transduction pathway. We named these proteins as small G protein signaling modulators (SGSM1/2/3). Northern blot analysis revealed that human SGSM2/3 are expressed ubiquitously in various tissues, whereas SGSM1 is expressed mainly in brain, heart, and testis. Mouse possessed the same protein family genes, and the in situ hybridization and immunohistochemical staining of tissue sections revealed that mouse Sgsm1/2/3 are expressed in the neurons of central nervous system, indicating the strong association of Sgsm family with neuronal function. Furthermore, endogenous Sgsm1 protein was localized in the trans-Golgi network of mouse Neuro2a cells by immunofluorescence microscopy. Expression of various cDNA constructs followed by immunoprecipitation assay revealed that human SGSM1/2/3 proteins are coprecipitated with RAP and RAB subfamily members of the small G protein superfamily. Based on these results, we postulated that the SGSM family members function as modulators of the small G protein RAP and RAB-mediated neuronal signal transduction and vesicular transportation pathways.
Amino Acid Motifs, Molecular Sequence Data, RUN motif, Transfection, SGSM, Mice, Genetics, Animals, Humans, Immunoprecipitation, Amino Acid Sequence, Cloning, Molecular, Vesicle transportation, Cells, Cultured, Phylogeny, Neurons, Models, Genetic, Intracellular Signaling Peptides and Proteins, Small GTPase RAB, Protein Structure, Tertiary, Chromosome 22, rap GTP-Binding Proteins, rab GTP-Binding Proteins, Intracellular signaling, Small GTPase RAP, TBC motif, Signal Transduction
Amino Acid Motifs, Molecular Sequence Data, RUN motif, Transfection, SGSM, Mice, Genetics, Animals, Humans, Immunoprecipitation, Amino Acid Sequence, Cloning, Molecular, Vesicle transportation, Cells, Cultured, Phylogeny, Neurons, Models, Genetic, Intracellular Signaling Peptides and Proteins, Small GTPase RAB, Protein Structure, Tertiary, Chromosome 22, rap GTP-Binding Proteins, rab GTP-Binding Proteins, Intracellular signaling, Small GTPase RAP, TBC motif, Signal Transduction
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).56 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!