Transformation ofClostridium thermohydrosulfuricum DSM 568 with plasmid DNA

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1985 English Publisher:Springer Science and Business Media LLCJournal:Biotechnology Letters, volume 7, pages 705-710 (issn: 0141-5492, eissn: 1573-6776,

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Authors: Erwin Soutschek-Bauer; Lydia Hartl; Walter L. Staudenbauer;

doi: 10.1007/bf01032280

Transformation ofClostridium thermohydrosulfuricum DSM 568 with plasmid DNA

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Abstract

A method was developed for the transformation of the ethanol-producing thermophilic bacteriumClostridiumthermohydrosulfuricum DSM 568 without protoplast formation. Competence for DNA uptake was induced by treatment ofCl.thermohydrosulfuricum cells with 50 mM Tris-HCl pH 8. 3. In the presence of polyethylene glycol (PEG 6000) the Tris-treated cells were transformed with the antibiotic resistance plasmids pUB110 (KmR) and pGS13 (KmR CmR) at frequencies of 4×10−6 per viable cell. This transformation method will be useful for the development of genetic exchange systems in thermophilic clostridia of biotechnological interest.

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Technical University of Munich
Germany

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