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FEBS Letters
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http://dx.doi.org/10.1016/0014...
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FEBS Letters
Article . 1989 . Peer-reviewed
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FEBS Letters
Article . 1989
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Some evidence of the enzymatic conversion of bovine suppressor phosphoseryl‐tRNA to selenocysteyl‐tRNA

descriptionPublicationkeyboard_double_arrow_right Article 03 Jul 1989 English Publisher:WileyJournal:FEBS Letters, volume 250, pages 142-146 (issn: 0014-5793, eissn: 1873-3468, Copyright policy )
Authors: Mizutani, Takaharu;

doi: 10.1016/0014-5793(89)80707-0

pmid: 2526748

Some evidence of the enzymatic conversion of bovine suppressor phosphoseryl‐tRNA to selenocysteyl‐tRNA

Abstract

In order to clarify the mechanisms of selenocysteine incorporation into glutathione peroxidase, some evidence to show the in vitro conversion of phosphoseryl‐tRNA to selenocysteyl‐tRNA is reported. [3H]Phosphoseryl‐tRNA was incubated in a reaction mixture composed of SeO2, glutathione and NADPH in the presence of selenium‐transferase partially purified. Analyses of amino acids on the product tRNA showed that a part (4%) of [3H]phosphoseryl‐tRNA was changed to [3H]selenocysteyl‐tRNA. The conversion from seryl‐tRNAsu or major seryl‐tRNAIGA was not found. Selenium‐transferase was essential for the conversion. [3H]Selenocysteine, liberated from the tRNA, was modified with iodoacetic acid. The product was confirmed to be carboxymethyl‐selenocysteine by two‐dimensional TLC. Selenocysteyl‐tRNAsu should be used to synthesize glutathione peroxidase by co‐translational mechanisms.

Related Organizations
Keywords

Opal nonsense codon, RNA, Transfer, Amino Acyl, Glutathione, Selenocysteine, Selenium, RNA, Transfer, Transferases, Glutathione peroxidase, Animals, Phosphoseryl-tRNA, Cattle, Chromatography, Thin Layer, Cysteine, tRNA, suppressor, Amino Acids, NADP

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    popularity
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
25
Average
Top 10%
Top 10%
bronze