Retinoic acid receptor‐related orphan receptor α regulates a subset of cone genes during mouse retinal development
Copyright policy )Retinoic acid receptor‐related orphan receptor α regulates a subset of cone genes during mouse retinal development
AbstractColor vision is supported by retinal cone photoreceptors that, in most mammals, express two photopigments sensitive to short (S‐opsin) or middle (M‐opsin) wavelengths. Expression of the Opn1sw and Opn1mw genes, encoding S‐opsin and M‐opsin, respectively, is under the control of nuclear receptors, including thyroid hormone receptor β2 (TRβ2), retinoid X receptor γ (RXRγ), and RORβ, a member of the retinoic acid receptor‐related orphan receptor (ROR) family. We now demonstrate that RORα, another member of the ROR family, regulates Opn1sw, Opn1mw, as well as Arr3 (cone arrestin) in the mouse retina. RORα expression is detected in cones by postnatal day 3 and maintained through adulthood. The retinas of staggerer mice, carrying a null mutation of RORα, show significant down‐regulation of Opn1sw, Opn1mw, and Arr3. RORα acts in synergy with cone‐rod homeobox transcription factor (Crx), to activate the Opn1sw promoter in vitro. Chromatin immunoprecipitation assays reveal that RORα directly binds to the Opn1sw promoter, Opn1mw locus control region, and the Arr3 promoter in vivo. Our data suggest that RORα plays a crucial role in cone development by directly regulating multiple cone genes.
- University of Ottawa Canada
- University of Toronto Canada
- University Health Network Canada
- Tokyo Women's Medical University Japan
- University of Ottawa Canada
Homeodomain Proteins, Mice, Knockout, Chromatin Immunoprecipitation, Arrestins, Rod Opsins, Down-Regulation, Gene Expression Regulation, Developmental, Receptors, Cytoplasmic and Nuclear, Nuclear Receptor Subfamily 1, Group F, Member 1, Embryo, Mammalian, Retina, Mice, Inbred C57BL, Mice, Mice, Neurologic Mutants, Basic-Leucine Zipper Transcription Factors, Animals, Newborn, Retinal Cone Photoreceptor Cells, Trans-Activators, Animals, Eye Proteins
Homeodomain Proteins, Mice, Knockout, Chromatin Immunoprecipitation, Arrestins, Rod Opsins, Down-Regulation, Gene Expression Regulation, Developmental, Receptors, Cytoplasmic and Nuclear, Nuclear Receptor Subfamily 1, Group F, Member 1, Embryo, Mammalian, Retina, Mice, Inbred C57BL, Mice, Mice, Neurologic Mutants, Basic-Leucine Zipper Transcription Factors, Animals, Newborn, Retinal Cone Photoreceptor Cells, Trans-Activators, Animals, Eye Proteins
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