Erythropoietin‐producing hepatocyte B6 variant‐derived peptides with the ability to induce glioma‐reactive cytotoxic T lymphocytes in human leukocyte antigen‐A2+ glioma patients
Copyright policy )Erythropoietin‐producing hepatocyte B6 variant‐derived peptides with the ability to induce glioma‐reactive cytotoxic T lymphocytes in human leukocyte antigen‐A2+ glioma patients
We recently cloned a variant form of erythropoietin‐producing hepatocyte (Eph)B6, a member of the Eph receptor tyrosine kinase family. In the present study, we examined the expression of the EphB6 variant (EphB6v) in a panel of brain tumor cell lines and glioblastoma tissues and we found that EphB6v was preferentially expressed in malignant brain tumors, such as glioblastomas and anaplastic astrocytomas. The EphB6v has a unique 54 amino acid sequence at the C‐terminal that is not found in normal EphB6. Therefore, we attempted to identify antigenic peptides unique to EphB6v for immunotherapy. The two EphB6v‐derived peptides exhibited the ability to bind to human leukocyte antigen (HLA)‐A0201 molecules, and each of them was able to induce cytotoxic T lymphocytes in vitro in the peripheral blood mononuclear cells of HLA‐A2+ glioma patients. The cytotoxicity was mediated by peptide‐specific CD8+ T cells in an HLA‐A2‐restricted manner. The expression of EphB6v was also observed in different types of cancer (e.g. lung, colon, stomach, liver and pancreatic) cells. Taken together, the two peptides derived from EphB6v might be appropriate targets for peptide‐based specific immunotherapy for HLA‐A2+ patients with various cancers. (Cancer Sci 2008; 99: 1656–1662)
- Kyushu Institute of Technology Japan
- Kurume University Japan
HLA-A Antigens, Brain Neoplasms, Gene Expression, Receptor Protein-Tyrosine Kinases, Astrocytoma, Gene Expression Regulation, Case-Control Studies, Cell Line, Tumor, HLA-A2 Antigen, Hepatocytes, Humans, Immunotherapy, Glioblastoma, Receptors, Eph Family, T-Lymphocytes, Cytotoxic
HLA-A Antigens, Brain Neoplasms, Gene Expression, Receptor Protein-Tyrosine Kinases, Astrocytoma, Gene Expression Regulation, Case-Control Studies, Cell Line, Tumor, HLA-A2 Antigen, Hepatocytes, Humans, Immunotherapy, Glioblastoma, Receptors, Eph Family, T-Lymphocytes, Cytotoxic
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!