Citron Kinase is an essential effector of the Pbl-activated Rho signalling pathway inDrosophila melanogaster
Copyright policy )Citron Kinase is an essential effector of the Pbl-activated Rho signalling pathway inDrosophila melanogaster
Pebble (Pbl)-activated RhoA signalling is essential for cytokinesis in Drosophila melanogaster. Here we report that the Drosophila citron gene encodes an essential effector kinase of Pbl-RhoA signalling in vivo. Drosophila citron is expressed in proliferating tissues but is downregulated in differentiating tissues. We find that Citron can bind RhoA and that localisation of Citron to the contractile ring is dependent on the cytokinesis-specific Pbl-RhoA signalling. Phenotypic analysis of mutants showed that citron is required for cytokinesis in every tissue examined, with mutant cells exhibiting multinucleate and hyperploid phenotypes. Strong genetic interactions were observed between citronand pbl alleles and constructs. Vertebrate studies implicate at least two Rho effector kinases, Citron and Rok, in cytokinesis. By contrast, we failed to find evidence for a role for the Drosophila ortholog of Rok in cell division. We conclude that Citron plays an essential, non-redundant role in the Rho signalling pathway during Drosophila cytokinesis.
- University of Adelaide Australia
- Australian National University Australia
cell division, 571, Keywords: gene product, cytokinesis, Protein Serine-Threonine Kinases, Pebble, Evolution, Molecular, Polyploidy, Citron Kinase, Rho, Sequence Analysis, Protein, insect protein, Animals, Drosophila Proteins, Guanine Nucleotide Exchange Factors, controlled study, Rho kinase, gene act Citron kinase, GEF, Rho Kinase, cell mutant, Conserved Sequence, Cytokinesis, Rho GTPase signalling, protein Rok, allele, article, Intracellular Signaling Peptides and Proteins, protein Citron, Brain, Cell Differentiation, guanosine triphosphatase, unclassified drug, Drosophila melanogaster, Rho factor, Mutation, protein pbl, Drosophila, rhoA GTP-Binding Protein, down regulation, Cell Division, Signal Transduction
cell division, 571, Keywords: gene product, cytokinesis, Protein Serine-Threonine Kinases, Pebble, Evolution, Molecular, Polyploidy, Citron Kinase, Rho, Sequence Analysis, Protein, insect protein, Animals, Drosophila Proteins, Guanine Nucleotide Exchange Factors, controlled study, Rho kinase, gene act Citron kinase, GEF, Rho Kinase, cell mutant, Conserved Sequence, Cytokinesis, Rho GTPase signalling, protein Rok, allele, article, Intracellular Signaling Peptides and Proteins, protein Citron, Brain, Cell Differentiation, guanosine triphosphatase, unclassified drug, Drosophila melanogaster, Rho factor, Mutation, protein pbl, Drosophila, rhoA GTP-Binding Protein, down regulation, Cell Division, Signal Transduction
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