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SSCP Analysis of cDNA Markers Provides a Dense Linkage Map of the Aedes aegypti Genome

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 2001 English Publisher:Oxford University Press (OUP)Journal:Genetics, volume 158, pages 715-726 (eissn: 1943-2631,

Authors: Ruth E. Fulton; Michael L. Salasek; William C. Black; Nancy M. DuTeau;

doi: 10.1093/genetics/158.2.715

pmid: 11404335

pmc: PMC1461678

SSCP Analysis of cDNA Markers Provides a Dense Linkage Map of the Aedes aegypti Genome

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Abstract

Abstract An intensive linkage map of the yellow fever mosquito, Aedes aegypti, was constructed using single-strand conformation polymorphism (SSCP) analysis of cDNA markers to identify single nucleotide polymorphisms (SNPs). A total of 94 A. aegypti cDNAs were downloaded from GenBank and primers were designed to amplify fragments <500 bp in size. These primer pairs amplified 94 loci, 57 (61%) of which segregated in a single F1 intercross family among 83 F2 progeny. This allowed us to produce a dense linkage map of one marker every 2 cM distributed over a total length of 134 cM. Many A. aegypti cDNAs were highly similar to genes in the Drosophila melanogaster genome project. Comparative linkage analysis revealed areas of synteny between the two species. SNP polymorphisms are abundant in A. aegypti genes and should prove useful in both population genetics and mapping studies.

Related Organizations

Colorado State University
United States

Keywords

Expressed Sequence Tags, Genetic Markers, DNA, Complementary, Genome, Base Sequence, Genotype, Genetic Linkage, Molecular Sequence Data, Chromosome Mapping, Aedes, Sequence Homology, Nucleic Acid, Animals, Crosses, Genetic, Polymorphism, Single-Stranded Conformational, Microsatellite Repeats

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