Cryo-EM structures of the human INO80 chromatin-remodeling complex
Copyright policy )Cryo-EM structures of the human INO80 chromatin-remodeling complex
Access to chromatin for processes such as transcription and DNA repair requires the sliding of nucleosomes along DNA. This process is aided by chromatin-remodeling complexes, such as the multisubunit INO80 chromatin-remodeling complex. Here we present cryo-EM structures of the active core complex of human INO80 at 9.6 Å, with portions at 4.1-Å resolution, and reconstructions of combinations of subunits. Together, these structures reveal the architecture of the INO80 complex, including Ino80 and actin-related proteins, which is assembled around a single RUVBL1 (Tip49a) and RUVBL2 (Tip49b) AAA+ heterohexamer. An unusual spoked-wheel structural domain of the Ino80 subunit is engulfed by this heterohexamer; both, in combination, form the core of the complex. We also identify a cleft in RUVBL1 and RUVBL2, which forms a major interaction site for partner proteins and probably communicates these interactions to its nucleotide-binding sites.
- Imperial College London United Kingdom
Models, Molecular, Biochemistry & Molecular Biology, Biophysics, Crystallography, X-Ray, Article, MECHANISMS, Databases, DOMAIN, Protein Domains, Models, BINDING, Humans, CRYSTAL-STRUCTURE, TRANSCRIPTION, Databases, Protein, 11 Medical and Health Sciences, ARCHITECTURE, Crystallography, Science & Technology, Binding Sites, Protein, Cryoelectron Microscopy, DNA Helicases, Molecular, 500, Cell Biology, 06 Biological Sciences, 540, Chromatin, Nucleosomes, DNA-Binding Proteins, ACTIN-RELATED PROTEINS, ALIGNMENT, RESOLUTION, X-Ray, ATPASE, ATPases Associated with Diverse Cellular Activities, Protein Multimerization, 03 Chemical Sciences, Carrier Proteins, Life Sciences & Biomedicine, Developmental Biology, Protein Binding
Models, Molecular, Biochemistry & Molecular Biology, Biophysics, Crystallography, X-Ray, Article, MECHANISMS, Databases, DOMAIN, Protein Domains, Models, BINDING, Humans, CRYSTAL-STRUCTURE, TRANSCRIPTION, Databases, Protein, 11 Medical and Health Sciences, ARCHITECTURE, Crystallography, Science & Technology, Binding Sites, Protein, Cryoelectron Microscopy, DNA Helicases, Molecular, 500, Cell Biology, 06 Biological Sciences, 540, Chromatin, Nucleosomes, DNA-Binding Proteins, ACTIN-RELATED PROTEINS, ALIGNMENT, RESOLUTION, X-Ray, ATPASE, ATPases Associated with Diverse Cellular Activities, Protein Multimerization, 03 Chemical Sciences, Carrier Proteins, Life Sciences & Biomedicine, Developmental Biology, Protein Binding
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