Identification of Genes That Function in the Biogenesis and Localization of Small Nucleolar RNAs in Saccharomyces cerevisiae
Copyright policy )Identification of Genes That Function in the Biogenesis and Localization of Small Nucleolar RNAs in Saccharomyces cerevisiae
Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently localize to the Cajal body (in plant and animal cells) or the homologous nucleolar body (in budding yeast) for maturation and assembly into snoRNPs prior to accumulation in their primary functional site, the nucleolus. However, little is known about the trans-acting factors important for the intranuclear trafficking and nucleolar localization of snoRNAs. Here, we describe a large-scale genetic screen to identify proteins important for snoRNA transport in Saccharomyces cerevisiae. We performed fluorescence in situ hybridization analysis to visualize U3 snoRNA localization in a collection of temperature-sensitive yeast mutants. We have identified Nop4, Prp21, Tao3, Sec14, and Htl1 as proteins important for the proper localization of U3 snoRNA. Mutations in genes encoding these proteins lead to specific defects in the targeting or retention of the snoRNA to either the nucleolar body or the nucleolus. Additional characterization of the mutants revealed impairment in specific steps of U3 snoRNA processing, demonstrating that snoRNA maturation and trafficking are linked processes.
- Carnegie Mellon University United States
- University of Liège Belgium
- Fund for Scientific Research Belgium
- University of Georgia Georgia
- Université Libre de Bruxelles Belgium
Hot Temperature, Saccharomyces cerevisiae Proteins, Ribonucleoproteins, Small Nucleolar/analysis/genetics/metabolism, Saccharomyces cerevisiae Proteins/analysis/genetics/metabolism, RNA, Fungal/analysis/metabolism, Genes, Fungal, Saccharomyces cerevisiae, Cell Nucleolus -- chemistry -- metabolism, Biochimie, biophysique & biologie moléculaire, Ribonucleoproteins, Small Nucleolar, RNA Precursors/metabolism, RNA Precursors, RNA, Small Nucleolar, Small Nucleolar -- analysis -- genetics -- metabolism, Small Nucleolar -- analysis -- metabolism, Nuclear Proteins/analysis/genetics/metabolism, Alleles, Saccharomyces cerevisiae -- genetics -- metabolism, synthèse des protéines, Biologie moléculaire, Nuclear Proteins, RNA, Fungal, RNA, Small Nucleolar/analysis/metabolism, Nuclear Proteins -- analysis -- genetics -- metabolism, Life sciences, Saccharomyces cerevisiae Proteins -- analysis -- genetics -- metabolism, Cell Nucleolus/chemistry/metabolism, Fungal, Genes, Ribonucleoproteins, Fungal -- analysis -- metabolism, FOS: Biological sciences, Mutation, Sciences du vivant, RNA, Biologie cellulaire, RNA Precursors -- metabolism, Saccharomyces cerevisiae/genetics/metabolism, Acides nucléiques, Cell Nucleolus, 69999 Biological Sciences not elsewhere classified, Biochemistry, biophysics & molecular biology
Hot Temperature, Saccharomyces cerevisiae Proteins, Ribonucleoproteins, Small Nucleolar/analysis/genetics/metabolism, Saccharomyces cerevisiae Proteins/analysis/genetics/metabolism, RNA, Fungal/analysis/metabolism, Genes, Fungal, Saccharomyces cerevisiae, Cell Nucleolus -- chemistry -- metabolism, Biochimie, biophysique & biologie moléculaire, Ribonucleoproteins, Small Nucleolar, RNA Precursors/metabolism, RNA Precursors, RNA, Small Nucleolar, Small Nucleolar -- analysis -- genetics -- metabolism, Small Nucleolar -- analysis -- metabolism, Nuclear Proteins/analysis/genetics/metabolism, Alleles, Saccharomyces cerevisiae -- genetics -- metabolism, synthèse des protéines, Biologie moléculaire, Nuclear Proteins, RNA, Fungal, RNA, Small Nucleolar/analysis/metabolism, Nuclear Proteins -- analysis -- genetics -- metabolism, Life sciences, Saccharomyces cerevisiae Proteins -- analysis -- genetics -- metabolism, Cell Nucleolus/chemistry/metabolism, Fungal, Genes, Ribonucleoproteins, Fungal -- analysis -- metabolism, FOS: Biological sciences, Mutation, Sciences du vivant, RNA, Biologie cellulaire, RNA Precursors -- metabolism, Saccharomyces cerevisiae/genetics/metabolism, Acides nucléiques, Cell Nucleolus, 69999 Biological Sciences not elsewhere classified, Biochemistry, biophysics & molecular biology
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