Role of the PLA2‐independent peroxiredoxin VI activity in the survival of immortalized fibroblasts exposed to cytotoxic oxidative stress
Copyright policy )Role of the PLA2‐independent peroxiredoxin VI activity in the survival of immortalized fibroblasts exposed to cytotoxic oxidative stress
Peroxiredoxin VI (PrxVI) is a bifunctional enzyme with non‐selenium glutathione peroxidase and Ca2+‐independent acidic phospholipase A2 activities. We demonstrate that transfection‐mediated PrxVI overexpression protects immortalized human WI‐38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert‐butylhydroperoxide and H2O2. Mutants for either glutathione peroxidase or phospholipase A2 activity show that glutathione peroxidase but not phospholipase A2 activity is required to promote cell survival after stress. Also, ectopic PrxVI overexpression does not protect telomerase‐stabilized WI‐38 fibroblasts against stress‐induced premature senescence.
- University of Namur Belgium
- Université de Namur Belgium
Cell Survival, Transfection, Gene Expression Regulation, Enzymologic, Phospholipases A, Mice, tert-Butylhydroperoxide, Animals, Humans, Premature senescence, Telomerase, Cellular Senescence, Peroxiredoxin, 3T3 Cells, Hydrogen Peroxide, Peroxiredoxins, Fibroblasts, Recombinant Proteins, Oxidative Stress, Phospholipases A2, Peroxidases, Oxidative stress, Fibroblast, Peroxiredoxin VI
Cell Survival, Transfection, Gene Expression Regulation, Enzymologic, Phospholipases A, Mice, tert-Butylhydroperoxide, Animals, Humans, Premature senescence, Telomerase, Cellular Senescence, Peroxiredoxin, 3T3 Cells, Hydrogen Peroxide, Peroxiredoxins, Fibroblasts, Recombinant Proteins, Oxidative Stress, Phospholipases A2, Peroxidases, Oxidative stress, Fibroblast, Peroxiredoxin VI
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