High‐Efficiency RNA Interference in Human Embryonic Stem Cells
Copyright policy )High‐Efficiency RNA Interference in Human Embryonic Stem Cells
RNA interference methodology suppresses gene expression, thus mimicking loss-of-function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high-efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell-specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
- University of Mary United States
- Boston Children's Hospital United States
- Technion – Israel Institute of Technology Israel
- Washington University in St. Louis United States
- Harvard Stem Cell Institute, Cambridge, MA, USA United States
Homeodomain Proteins, Pluripotent Stem Cells, Genetic Vectors, Green Fluorescent Proteins, Lentivirus, Gene Expression, Gene Expression Regulation, Developmental, Cell Differentiation, Nanog Homeobox Protein, Embryo, Mammalian, Cell Line, DNA-Binding Proteins, Glycoprotein Hormones, alpha Subunit, Antigens, Surface, Humans, Proteoglycans, RNA Interference, RNA, Small Interfering, Octamer Transcription Factor-3, Glycoproteins
Homeodomain Proteins, Pluripotent Stem Cells, Genetic Vectors, Green Fluorescent Proteins, Lentivirus, Gene Expression, Gene Expression Regulation, Developmental, Cell Differentiation, Nanog Homeobox Protein, Embryo, Mammalian, Cell Line, DNA-Binding Proteins, Glycoprotein Hormones, alpha Subunit, Antigens, Surface, Humans, Proteoglycans, RNA Interference, RNA, Small Interfering, Octamer Transcription Factor-3, Glycoproteins
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