Type 3 and Type 1 Ryanodine Receptors Are Localized in Triads of the Same Mammalian Skeletal Muscle Fibers
Copyright policy )Type 3 and Type 1 Ryanodine Receptors Are Localized in Triads of the Same Mammalian Skeletal Muscle Fibers
The type 3 ryanodine receptor (RyR3) is a ubiquitous calcium release channel that has recently been found in mammalian skeletal muscles. However, in contrast to the skeletal muscle isoform (RyR1), neither the subcellular distribution nor the physiological role of RyR3 are known. Here, we used isoform-specific antibodies to localize RyR3 in muscles of normal and RyR knockout mice. In normal hind limb and diaphragm muscles of young mice, RyR3 was expressed in all fibers where it was codistributed with RyR1 and with the skeletal muscle dihydropyridine receptor. This distribution pattern indicates that RyR3 is localized in the triadic junctions between the transverse tubules and the sarcoplasmic reticulum. During development, RyR3 expression declined rapidly in some fibers whereas other fibers maintained expression of RyR3 into adulthood. Comparing the distribution of RyR3-containing fibers with that of known fiber types did not show a direct correlation. Targeted deletion of the RyR1 or RyR3 gene resulted in the expected loss of the targeted isoform, but had no adverse effects on the expression and localization of the respective other RyR isoform. The localization of RyR3 in skeletal muscle triads, together with RyR1, is consistent with an accessory function of RyR3 in skeletal muscle excitation–contraction coupling.
- University of Tokyo Japan
- University of Innsbruck Austria
- University of Siena Italy
Aging, Calcium Channels, L-Type, Knockout, Blotting, Western, Diaphragm, Muscle Fibers, Skeletal, cytology/embryology/metabolism/ultrastructure, genetics/metabolism, Calcium-Transporting ATPase, Down-Regulation, Fluorescent Antibody Technique, Calcium-Transporting ATPases, Calcium Channel, Mice, Animals, Protein Isoforms, Developmental, Muscle, Skeletal, cytology/metabolism, Mice, Knockout, Myosin Heavy Chains, Animal, Blotting, Myosin Heavy Chain, Protein Isoform, Gene Expression Regulation, Developmental, Ryanodine Receptor Calcium Release Channel, Skeletal, Newborn, enzymology/metabolism, L-Type, Hindlimb, Muscle Fiber, Sarcoplasmic Reticulum, Gene Expression Regulation, Animals, Newborn, Muscle, Calcium Channels, Western, metabolism, Aging, Animals, Animals; Newborn, Blotting; Western, Calcium Channels; L-Type, Calcium Channels; metabolism, Calcium-Transporting ATPases; metabolism, Diaphragm; cytology/metabolism, Down-Regulation, Fluorescent Antibody Technique, Gene Deletion, Gene Expression Regulation; Developmental, Hindlimb; cytology/metabolism, Mice, Mice; Knockout, Muscle Fibers; Skeletal; enzymology/metabolism, Muscle; cytology/embryology/metabolism/ultrastructure, Myosin Heavy Chains; metabolism, Protein Isoforms; genetics/metabolism, Ryanodine Receptor Calcium Release Channel; genetics/metabolism, Sarcoplasmic Reticulum; enzymology/metabolism, Gene Deletion
Aging, Calcium Channels, L-Type, Knockout, Blotting, Western, Diaphragm, Muscle Fibers, Skeletal, cytology/embryology/metabolism/ultrastructure, genetics/metabolism, Calcium-Transporting ATPase, Down-Regulation, Fluorescent Antibody Technique, Calcium-Transporting ATPases, Calcium Channel, Mice, Animals, Protein Isoforms, Developmental, Muscle, Skeletal, cytology/metabolism, Mice, Knockout, Myosin Heavy Chains, Animal, Blotting, Myosin Heavy Chain, Protein Isoform, Gene Expression Regulation, Developmental, Ryanodine Receptor Calcium Release Channel, Skeletal, Newborn, enzymology/metabolism, L-Type, Hindlimb, Muscle Fiber, Sarcoplasmic Reticulum, Gene Expression Regulation, Animals, Newborn, Muscle, Calcium Channels, Western, metabolism, Aging, Animals, Animals; Newborn, Blotting; Western, Calcium Channels; L-Type, Calcium Channels; metabolism, Calcium-Transporting ATPases; metabolism, Diaphragm; cytology/metabolism, Down-Regulation, Fluorescent Antibody Technique, Gene Deletion, Gene Expression Regulation; Developmental, Hindlimb; cytology/metabolism, Mice, Mice; Knockout, Muscle Fibers; Skeletal; enzymology/metabolism, Muscle; cytology/embryology/metabolism/ultrastructure, Myosin Heavy Chains; metabolism, Protein Isoforms; genetics/metabolism, Ryanodine Receptor Calcium Release Channel; genetics/metabolism, Sarcoplasmic Reticulum; enzymology/metabolism, Gene Deletion
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