(A) A404 cells were infected with adenovirus expressing Pitx2a or empty adenovirus, and acetylation levels of histone H4 at the promoter region of the gene were determined by ChIP assays

Name: (A) A404 cells were infected with adenovirus expressing Pitx2a or empty adenovirus, and acetylation levels of histone H4 at the promoter region of the gene were determined by ChIP assays
Keywords: 3. Good health

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2011Publisher:figshare

Authors: Yueting Shang; Yoshida, Tadashi; Amendt, Brad A.; Martin, James F.; Owens, Gary K.;

doi: 10.6084/m9.figshare.79232 , 10.6084/m9.figshare.79232.v1

(A) A404 cells were infected with adenovirus expressing Pitx2a or empty adenovirus, and acetylation levels of histone H4 at the promoter region of the gene were determined by ChIP assays

- Summary
- Related research
  (9)
- Metrics

Abstract

(B) Luciferase assays were performed in A404 cells cotransfected with HDAC expression plasmids, Pitx2a expression plasmid, and the promoter-enhancer-luciferase construct. (C, left) Efficiency of siRNA was tested by Western blotting. (C, right) Luciferase assays were performed in A404 cells cotransfected with Pitx2a expression plasmid, expression plasmid for p300 or siRNA, and the promoter-enhancer-luciferase construct. (D) A404 cells were infected with adenovirus expressing Pitx2a or empty adenovirus, and the association of p300, HDAC2, and HDAC5 with the CArG-containing region of the gene was determined by ChIP assays. Values represent the mean ± SEM of three independent experiments.Copyright information:Taken from "Pitx2 is functionally important in the early stages of vascular smooth muscle cell differentiation"The Journal of Cell Biology 2008;181(3):461-473.Published online 5 May 2008PMCID:PMC2364692.

9 Research products, page 1 of 1

Recruitment of Serum Response Factor and Hyperacetylation of Histones at Smooth Muscle–Specific Regulatory Regions During Differentiation of a Novel P19-Derived In Vitro Smooth Muscle Differentiation System
2001IsAmongTopNSimilarDocuments
Chromatin Remodeling Pathways in Smooth Muscle Cell Differentiation, and Evidence for an Integral Role for p300
2010IsAmongTopNSimilarDocuments
LPP Expression During In Vitro Smooth Muscle Differentiation and Stent-Induced Vascular Injury
2006IsAmongTopNSimilarDocuments
Pollen analysis - Montréal-la-Cluse (F-01), A404 derrière la côte du château
2017IsAmongTopNSimilarDocuments
(A) Luciferase assays were performed in A404 cells cotransfected with expression plasmids for Pitx2a and SRF and the promoter-enhancer-luciferase construct
2011IsAmongTopNSimilarDocuments
(A–C) A404 cells were induced to differentiate into SMCs by treatment with 1 μg/ml RA, followed by 0
2011IsAmongTopNSimilarDocuments
(A) Schematic structure of the promoter
2011IsAmongTopNSimilarDocuments
The Actin Associated Protein Palladin Is Important for the Early Smooth Muscle Cell Differentiation
2010IsAmongTopNSimilarDocuments
Transcriptional profiling of in vitro smooth muscle cell differentiation identifies specific patterns of gene and pathway activation
2004IsAmongTopNSimilarDocuments

Impact byBIP!

	selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	0
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average

Found an issue? Give us feedback

Average

Beta

SDGs Suggest

3. Good health

Beta

SDGs:

3. Good health,

Related to Research communities

Knowmad Institut