The IGF system in in-vitro human decidualization
Copyright policy )The IGF system in in-vitro human decidualization
Decidualization of endometrial stromal cells (ESCs) is critical for a successful pregnancy but the molecular mechanisms of the process are poorly understood. In this study, we investigated whether the insulin-like growth factor (IGF) network is involved in this cellular process. Expression kinetics of members of the IGF system was examined at both mRNA and protein levels during in-vitro decidualization of cultured human ESCs. We found a significant up-regulation of IGF-II as well as of IGF-I receptor and the A and B insulin receptor (InsR) isoforms. In addition, levels of the key adaptor proteins insulin receptor substrate 1 (IRS-1) and IRS-2 increased, suggesting a potential involvement of the IGF signalling pathway in the decidualization process. Expression of two IGF binding proteins, IGFBP-1 and IGFBP-4, which can inhibit IGF action, also increased. In order to determine whether IGF signalling was activated during decidualization, the phosphorylation status of the receptors and the adaptor proteins was estimated. Only IRS-2 was slightly phosphorylated in decidualized cells and was further activated by the addition of exogenous IGF-II. These results suggest that the IGF signalling pathway could play a crucial role in the functions of decidualized endometrial cells.
- University of Liège Belgium
- Université de Liège (ULiège) Belgium
- Université Libre de Bruxelles Belgium
- Université de Liège (University of Liège) Belgium
- University of Liege (ULG) Belgium
Adult, Endometrium -- cytology -- metabolism, Cells, Blotting, Western, progesterone, Anatomy (cytology, histology, embryology...) & physiology, Insulin-Like Growth Factor II -- genetics -- metabolism, Receptor, IGF Type 1, insulin-like growth factor, Endometrium, decidualization, Insulin-Like Growth Factor II, Somatomedins, cAMP, Stromal Cells -- cytology -- metabolism, Humans, Insulin-Like Growth Factor Binding Protein 1 -- genetics -- metabolism, IGF Type 1 -- genetics -- metabolism, Insulin -- genetics -- metabolism, Insulin-Like Growth Factor Binding Protein 4 -- genetics -- metabolism, endometrium, Phosphorylation, Cells, Cultured, Somatomedins -- genetics -- metabolism, Anatomie (cytologie, histologie, embryologie...) & physiologie, Cultured, Blotting, Reverse Transcriptase Polymerase Chain Reaction, Life sciences, Sciences biomédicales, Receptor, Insulin, Signal Transduction -- genetics -- physiology, Insulin-Like Growth Factor Binding Protein 1, Insulin-Like Growth Factor Binding Protein 4, Insulin Receptor Substrate Proteins -- genetics -- metabolism, Sciences du vivant, Insulin Receptor Substrate Proteins, Female, Stromal Cells, Western, Receptor, Signal Transduction
Adult, Endometrium -- cytology -- metabolism, Cells, Blotting, Western, progesterone, Anatomy (cytology, histology, embryology...) & physiology, Insulin-Like Growth Factor II -- genetics -- metabolism, Receptor, IGF Type 1, insulin-like growth factor, Endometrium, decidualization, Insulin-Like Growth Factor II, Somatomedins, cAMP, Stromal Cells -- cytology -- metabolism, Humans, Insulin-Like Growth Factor Binding Protein 1 -- genetics -- metabolism, IGF Type 1 -- genetics -- metabolism, Insulin -- genetics -- metabolism, Insulin-Like Growth Factor Binding Protein 4 -- genetics -- metabolism, endometrium, Phosphorylation, Cells, Cultured, Somatomedins -- genetics -- metabolism, Anatomie (cytologie, histologie, embryologie...) & physiologie, Cultured, Blotting, Reverse Transcriptase Polymerase Chain Reaction, Life sciences, Sciences biomédicales, Receptor, Insulin, Signal Transduction -- genetics -- physiology, Insulin-Like Growth Factor Binding Protein 1, Insulin-Like Growth Factor Binding Protein 4, Insulin Receptor Substrate Proteins -- genetics -- metabolism, Sciences du vivant, Insulin Receptor Substrate Proteins, Female, Stromal Cells, Western, Receptor, Signal Transduction
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