Protein Crystallization and Biosensor Applications of Hydrogel-Based Molecularly Imprinted Polymers
Copyright policy )Protein Crystallization and Biosensor Applications of Hydrogel-Based Molecularly Imprinted Polymers
We have characterized the imprinting capability of a family of acrylamide polymer-based molecularly imprinted polymers (MIPs) for bovine hemoglobin (BHb) and trypsin (Tryp) using spectrophotometric and quartz crystal microbalance (QCM) sensor techniques. Bulk gel characterization on acrylamide (AA), N-hydroxymethylacrylamide (NHMA), and N-isopropylacrylamide (NiPAM) gave varied selectivities when compared with nonimprinted polymers. We have also harnessed the ability of the MIPs to facilitate protein crystallization as a means of evaluating their selectivity for cognate and noncognate proteins. Crystallization trials indicated improved crystal formation in the order NiPAM
- Imperial College London United Kingdom
- University of Surrey United Kingdom
Acrylamides, Polymers, Protein Conformation, 500, Hydrogels, Biosensing Techniques, Molecular Imprinting, Hemoglobins, Quartz Crystal Microbalance Techniques, Animals, Cattle, Trypsin, Crystallization
Acrylamides, Polymers, Protein Conformation, 500, Hydrogels, Biosensing Techniques, Molecular Imprinting, Hemoglobins, Quartz Crystal Microbalance Techniques, Animals, Cattle, Trypsin, Crystallization
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