Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase fromMus musculusandHomo sapiens
Copyright policy )Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase fromMus musculusandHomo sapiens
Inositol monophosphatase (IMPase) catalyses the hydrolysis of inositol monophosphate to inositol and is crucial in the phosphatidylinositol (PI) signalling pathway. Lithium, which is the drug of choice for bipolar disorder, inhibits IMPase at therapeutically relevant plasma concentrations. Both mouse IMPase 1 (MmIMPase 1) and human IMPase 1 (HsIMPase 1) were cloned into pRSET5a, expressed in Escherichia coli, purified and crystallized using the sitting-drop method. The structures were solved at resolutions of 2.4 and 1.7 Å, respectively. Comparison of MmIMPase 1 and HsIMPase 1 revealed a core r.m.s. deviation of 0.516 Å.
- King's College London United Kingdom
- University of Oxford United Kingdom
- Koç University Turkey
- Kings College London, University of London United Kingdom
Models, Molecular, Protein Structure, Biochemistry and molecular biology; Biophysics, Inositol Phosphates, Biophysics, Gene Expression, Crystallography, X-Ray, Mice, Models, Animals, Humans, Myoinositol monophosphatase, Cloning, Molecular, bipolar disorder, Crystallography, Putative target, Molecular, 540, Lithium-theraphy, Crystallography; Inositol monophosphatase; lithium; bipolar disorder; Myoinositol monophosphatase; Lithium-theraphy; Putative target; Bovine brain; Enzyme, Protein Structure, Tertiary, lithium, Enzyme, Bovine brain, X-Ray, Inositol monophosphatase, Biochemistry and molecular biology, Crystallization, Tertiary, Cloning
Models, Molecular, Protein Structure, Biochemistry and molecular biology; Biophysics, Inositol Phosphates, Biophysics, Gene Expression, Crystallography, X-Ray, Mice, Models, Animals, Humans, Myoinositol monophosphatase, Cloning, Molecular, bipolar disorder, Crystallography, Putative target, Molecular, 540, Lithium-theraphy, Crystallography; Inositol monophosphatase; lithium; bipolar disorder; Myoinositol monophosphatase; Lithium-theraphy; Putative target; Bovine brain; Enzyme, Protein Structure, Tertiary, lithium, Enzyme, Bovine brain, X-Ray, Inositol monophosphatase, Biochemistry and molecular biology, Crystallization, Tertiary, Cloning
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