Biological evaluation, docking studies, and in silico ADME prediction of some pyrimidine and pyridine derivatives as potential EGFR WT and EGFR T790M inhibitors
Copyright policy )Biological evaluation, docking studies, and in silico ADME prediction of some pyrimidine and pyridine derivatives as potential EGFR WT and EGFR T790M inhibitors
Herein, a set of pyridine and pyrimidine derivatives were assessed for their impact on the cell cycle and apoptosis. Human breast cancer (MCF7), hepatocellular carcinoma (HEPG2), larynx cancer (HEP2), lung cancer (H460), colon cancers (HCT116 and Caco2), and hypopharyngeal cancer (FADU), and normal Vero cell lines were used. Compounds 8 and 14 displayed outstanding effects on the investigated cell lines and were further tested for their antioxidant activity in MCF7, H460, FADU, HEP2, HEPG2, HCT116, Caco2, and Vero cells by measuring superoxide dismutase (SOD), malondialdehyde content (MDA), reduced glutathione (GSH), and nitric oxide (NO) content. Besides, Annexin V-FITC apoptosis detection and cell cycle DNA index using the HEPG-2 cell line were established on both compounds as well. Furthermore, compounds 8 and 14 were assessed for their EGFR kinase (Wild and T790M) inhibitory activities, revealing eligible potential. Additionally, molecular docking, ADME, and SAR studies were carried out for the investigated candidates.
- Ahram Canadian University Egypt
- Kafrelsheikh University Egypt
- Cairo University Egypt
- National Institute for Materials Science Japan
- Princess Nourah bint Abdulrahman University Saudi Arabia
Lung Neoplasms, Pyridines, EGFR, Antineoplastic Agents, RM1-950, Structure-Activity Relationship, Cell Line, Tumor, Pyridine/pyrimidine derivatives, Chlorocebus aethiops, Animals, Humans, in vitro, Protein Kinase Inhibitors, Vero Cells, Cell Proliferation, Molecular Structure, ErbB Receptors, Molecular Docking Simulation, Pyrimidines, in silico, Mutation, Therapeutics. Pharmacology, Drug Screening Assays, Antitumor, Caco-2 Cells, Research Paper
Lung Neoplasms, Pyridines, EGFR, Antineoplastic Agents, RM1-950, Structure-Activity Relationship, Cell Line, Tumor, Pyridine/pyrimidine derivatives, Chlorocebus aethiops, Animals, Humans, in vitro, Protein Kinase Inhibitors, Vero Cells, Cell Proliferation, Molecular Structure, ErbB Receptors, Molecular Docking Simulation, Pyrimidines, in silico, Mutation, Therapeutics. Pharmacology, Drug Screening Assays, Antitumor, Caco-2 Cells, Research Paper
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