3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts.
Copyright policy )3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts.
The SH3 binding protein, 3BP-1, was originally cloned as a partial cDNA from an expression library using the Abl SH3 domain as a probe. In addition to an SH3 binding domain, 3BP-1 displayed homology to a class of GTPase activating proteins (GAPs) active against Rac and Rho proteins. We report here a full length cDNA of 3BP-1 which extends the homology to GAP proteins previously noted. 3BP-1 functions in vitro as a GAP with a specificity for Rac-related G proteins. Microinjection of the 3BP-1 protein into serum-starved fibroblasts produces an inhibition of platelet-derived growth factor (PDGF)-induced membrane ruffling mediated by Rac. Co-injection of 3BP-1 with an activated Rac mutant that is unresponsive to GAPs, counter-acts this inhibition. 3BP-1 does not show in vitro activity towards Rho and, in agreement with this finding, microinjection of 3BP-1 into fibroblasts has no effect on lysophosphatidic acid (LPA)-induced stress fiber assembly mediated by Rho. Thus 3BP-1 is a new and specific Rac GAP that can act in cells to counter Rac-mediated membrane ruffling. How its SH3 binding site interacts with its GAP activity remains to be understood.
- Rockefeller University United States
- California Institute of Technology United States
- Rockefeller University United States
570, Molecular Sequence Data, 3BP-I, 610, Phosphatidic Acids, Mice, GTP-Binding Proteins, Animals, Amino Acid Sequence, Growth Substances, Oncogene Proteins, Base Sequence, Cell Membrane, GTPase-Activating Proteins, cytoskeleton, GAP, 3T3 Cells, Fibroblasts, Actins, Rac, SH3, Genes, src, Gene Expression Regulation, Mutation, Guanosine Triphosphate, Lysophospholipids, Carrier Proteins
570, Molecular Sequence Data, 3BP-I, 610, Phosphatidic Acids, Mice, GTP-Binding Proteins, Animals, Amino Acid Sequence, Growth Substances, Oncogene Proteins, Base Sequence, Cell Membrane, GTPase-Activating Proteins, cytoskeleton, GAP, 3T3 Cells, Fibroblasts, Actins, Rac, SH3, Genes, src, Gene Expression Regulation, Mutation, Guanosine Triphosphate, Lysophospholipids, Carrier Proteins
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).70 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!