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Towards a More Precise Serological Diagnosis of Human Tegumentary Leishmaniasis Using Leishmania Recombinant Proteins

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 12 Jun 2013 Spain English Publisher:Public Library of Science (PLoS)Journal:PLoS ONE, volume 8, page e66110 (eissn: 1932-6203,

Authors: Manoel Barral-Netto; Manoel Barral-Netto; Manuel Soto; Ana Paula de Souza; Aldina Barral; Aldina Barral; Juqueline R. Cristal; +5 Authors

doi: 10.1371/journal.pone.0066110

pmid: 23776617

pmc: PMC3680450

handle: 10261/99588

Towards a More Precise Serological Diagnosis of Human Tegumentary Leishmaniasis Using Leishmania Recombinant Proteins

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Abstract

Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure.Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA.Our results raise possibility of using rHSP70 for the serodiagnosis of TL.

Country

Spain

Related Organizations

Autonomous University of Madrid
Spain
Spanish National Research Council
Spain
Centro de Biología Molecular Severo Ochoa
Spain
Oswaldo Cruz Foundation
Brazil
Federal University of Western Bahia
Brazil

Keywords

Leishmania, Science, Q, R, Oligonucleotides, Protozoan Proteins, Enzyme-Linked Immunosorbent Assay, Recombinant Proteins, Statistics, Nonparametric, ROC Curve, Medicine, Humans, HSP70 Heat-Shock Proteins, Serologic Tests, Leishmaniasis, Research Article

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