Upf1p, Nmd2p, and Upf3p Regulate the Decapping and Exonucleolytic Degradation of both Nonsense-Containing mRNAs and Wild-Type mRNAs
Copyright policy )Upf1p, Nmd2p, and Upf3p Regulate the Decapping and Exonucleolytic Degradation of both Nonsense-Containing mRNAs and Wild-Type mRNAs
In Saccharomyces cerevisiae, rapid degradation of nonsense-containing mRNAs requires the decapping enzyme Dcp1p, the 5'-to-3' exoribonuclease Xrn1p, and the three nonsense-mediated mRNA decay (NMD) factors, Upf1p, Nmd2p, and Upf3p. To identify specific functions for the NMD factors, we analyzed the mRNA decay phenotypes of yeast strains containing deletions of DCP1 or XRN1 and UPF1, NMD2, or UPF3. Our results indicate that Upf1p, Nmd2p, and Upf3p regulate decapping and exonucleolytic degradation of nonsense-containing mRNAs. In addition, we show that these factors also regulate the same processes in the degradation of wild-type mRNAs. The participation of the NMD factors in general mRNA degradation suggests that they may regulate an aspect of translation termination common to all transcripts.
- University of Massachusetts Medical School United States
RNA Caps, *RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Messenger, Saccharomyces cerevisiae, Fungal Proteins, Gene Expression Regulation, Fungal, Medicine and Health Sciences, Northern, RNA, Messenger, Alleles, Adaptor Proteins, Signal Transducing, Blotting, Life Sciences, RNA-Binding Proteins, Blotting, Northern, *Saccharomyces cerevisiae Proteins, Fungal, Phenotype, Gene Expression Regulation, Protein Biosynthesis, Exoribonucleases, Mutation, Trans-Activators, RNA, Gene Deletion, RNA Helicases
RNA Caps, *RNA-Binding Proteins, Saccharomyces cerevisiae Proteins, Messenger, Saccharomyces cerevisiae, Fungal Proteins, Gene Expression Regulation, Fungal, Medicine and Health Sciences, Northern, RNA, Messenger, Alleles, Adaptor Proteins, Signal Transducing, Blotting, Life Sciences, RNA-Binding Proteins, Blotting, Northern, *Saccharomyces cerevisiae Proteins, Fungal, Phenotype, Gene Expression Regulation, Protein Biosynthesis, Exoribonucleases, Mutation, Trans-Activators, RNA, Gene Deletion, RNA Helicases
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