Characterization of a functional neuropeptide F receptor from Drosophila melanogaster
Copyright policy )Characterization of a functional neuropeptide F receptor from Drosophila melanogaster
Potential receptors for Drosophila neuropeptide F (DmNPF) were identified in the genome database. One receptor (DmNPFR1) sequence resembled the Lymnaea NPY receptor, an invertebrate homolog of the vertebrate Y-receptor family. DmNPFR1 was cloned and tested for functionality in stably transfected mammalian CHO cells. In whole cell binding assays, DmNPF displaced 125I-NPF in a concentration-dependent manner (IC(50) = 65 nM). DmNPF inhibited forskolin-stimulated adenylyl cyclase activity similarly (IC(50) = 51 nM). Whole-mount in situ hybridization revealed that DmNPFR1 RNA is expressed in CNS and midgut of Drosophila larvae. DmNPFR1, a new invertebrate Y-receptor homolog, apparently is a functional receptor for DmNPF.
- University of Georgia Georgia
- University of Georgia Press United States
Receptors, Neuropeptide, Base Sequence, Molecular Sequence Data, Neuropeptides, Receptors, Cell Surface, CHO Cells, Receptors, Neuropeptide Y, Drosophila melanogaster, Cricetinae, Larva, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Nerve Tissue, Sequence Alignment, In Situ Hybridization, Phylogeny, Adenylyl Cyclases
Receptors, Neuropeptide, Base Sequence, Molecular Sequence Data, Neuropeptides, Receptors, Cell Surface, CHO Cells, Receptors, Neuropeptide Y, Drosophila melanogaster, Cricetinae, Larva, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Nerve Tissue, Sequence Alignment, In Situ Hybridization, Phylogeny, Adenylyl Cyclases
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