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(A) TM5b alone

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2011Publisher:figshare

Authors: Noda, Yumi; Horikawa, Saburo; Kanda, Eiichiro; Yamashita, Maho; Meng, Hu; Eto, Kayoko; Li, Yuhua; +6 Authors

doi: 10.6084/m9.figshare.28084 , 10.6084/m9.figshare.28084.v1

(A) TM5b alone

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Abstract

(B) TM5b incubated with F-actin was mixed with liposomes. (C–G) TM5b incubated with F-actin was mixed with liposomes reconstituted with Trx (C), nonphosphorylated AQP2 (D, F, and G), or phosphorylated AQP2 (E). Samples were fractionated on density gradient and immunoblotted for TM5b (A–E), actin (F), or AQP2 (G). Collected fractions were numbered from low (0% OptiPrep) to high (40% OptiPrep) density. (H) Relative densitometric quantification of TM5b in fractions 11–13 to total TM5b. Data represent the mean and SE from three independent experiments. *, P < 0.01 versus nonphosphorylated AQP2 liposome.Copyright information:Taken from "Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking"The Journal of Cell Biology 2008;182(3):587-601.Published online 11 Aug 2008PMCID:PMC2500142.

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