Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
Copyright policy )Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins
Division Machinery Tagged An international consortium of labs has been testing the feasibility of large-scale screening for insights into the function of mammalian proteins by expressing a tagged version of proteins from bacterial artificial chromosomes harbored in mammalian cells. Depending on the tag used, Hutchins et al. (p. 593 , published online 1 April) were able to monitor localization of tagged proteins by microscopy or to isolate interacting proteins and subsequently identify the binding partners by mass spectrometry. Applying the technology to proteins implicated in control of cell division revealed about 100 protein machines required for mitosis.
- Helmholtz Association of German Research Centres Germany
- University of Göttingen Germany
- Max Planck Society Germany
- University of Oxford United Kingdom
- Lunenfeld-Tanenbaum Research Institute Canada
Centrosome, Chromosomes, Artificial, Bacterial, Green Fluorescent Proteins, Mitosis, Ubiquitin-Protein Ligase Complexes, Genomics, Spindle Apparatus, Anaphase-Promoting Complex-Cyclosome, Open Reading Frames, Protein Subunits, Tubulin, Chromosome Segregation, Multiprotein Complexes, Databases, Genetic, Protein Interaction Mapping, Humans, RNA Interference, HeLa Cells, Protein Binding
Centrosome, Chromosomes, Artificial, Bacterial, Green Fluorescent Proteins, Mitosis, Ubiquitin-Protein Ligase Complexes, Genomics, Spindle Apparatus, Anaphase-Promoting Complex-Cyclosome, Open Reading Frames, Protein Subunits, Tubulin, Chromosome Segregation, Multiprotein Complexes, Databases, Genetic, Protein Interaction Mapping, Humans, RNA Interference, HeLa Cells, Protein Binding
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