p62/IMP2 stimulates cell migration and reduces cell adhesion in breast cancer
Copyright policy )p62/IMP2 stimulates cell migration and reduces cell adhesion in breast cancer
p62/IMP2 is an oncofetal protein that is overexpressed in several types of cancer, and is a member of the family of insulin-like growth factor 2 mRNA binding proteins. We previously reported that high levels of p62/IMP2 autoantibody are present in sera from cancer patients, compared to healthy individuals. Here, we report the overexpression of p62/IMP2 in tumor tissues of 72 out of 104 cases of human breast cancer, and high levels of p62/IMP2 autoantibody in patients' sera (in 63 out of 216 cases). To explore the role of p62/IMP2 in breast cancer progression, we generated p62/IMP2 transfected variants of two human breast cancer cell lines: MDA-MB-231 and LM2-4. Using in vitro assays we found that overexpression of p62/IMP2 can increase cell migration, and reduce cell adhesion to extracellular matrix (ECM) proteins. A Human Extracellular Matrix and Adhesion Molecules qPCR array was performed with our generated variants, and it identified a group of mRNAs whose expression was altered with p62/IMP2 overexpression, including connective tissue growth factor (CTGF) mRNA - which we show to be a p62/IMP2 binding partner. Overall, our results provide new insights into the molecular mechanism by which p62/IMP2 can contribute to breast cancer progression.
- The University of Texas System United States
- The University of Texas at El Paso United States
- UNIVERSITY OF TEXAS EL PASO
IGF2 mRNA binding protein 2, 570, Time Factors, cell migration, extracellular matrix, RNA Stability, 610, Breast Neoplasms, Transfection, Polymerase Chain Reaction, Proto-Oncogene Proteins c-myc, breast cancer, Cell Movement, Cell Line, Tumor, Cell Adhesion, Humans, RNA, Messenger, Biology, Autoantibodies, Gene Expression Profiling, Connective Tissue Growth Factor, RNA-Binding Proteins, cell adhesion, Up-Regulation, Gene Expression Regulation, Neoplastic, Female, Protein Binding, Signal Transduction
IGF2 mRNA binding protein 2, 570, Time Factors, cell migration, extracellular matrix, RNA Stability, 610, Breast Neoplasms, Transfection, Polymerase Chain Reaction, Proto-Oncogene Proteins c-myc, breast cancer, Cell Movement, Cell Line, Tumor, Cell Adhesion, Humans, RNA, Messenger, Biology, Autoantibodies, Gene Expression Profiling, Connective Tissue Growth Factor, RNA-Binding Proteins, cell adhesion, Up-Regulation, Gene Expression Regulation, Neoplastic, Female, Protein Binding, Signal Transduction
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