Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI.
Copyright policy )Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI.
Binding of a multiprotein complex to a 5' exon inhibitory element appears to repress splicing of the Drosophila P-element third intron (IVS3) in the soma. We have purified 97- and 50-kD proteins that interact specifically with the inhibitory element using RNA affinity chromatography. Antibodies specific for the 97-kD protein relieve inhibition of IVS3 splicing in somatic extracts, providing direct evidence that inhibition requires this protein, P-element somatic inhibitor (PSI). We identify the 50-kD protein as hrp48, a protein similar to the mammalian splicing factor hnRNP A1, and show that hrp48 recognizes specific nucleotides in a pseudo-5' splice site within the inhibitory element. The results indicate that PSI is an alternative splicing factor that regulates tissue-specific splicing, probably through interactions with generally expressed factors such as hrp48.
- University of California, Berkeley United States
Mammals, Base Sequence, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, Gene Expression, Exons, Chromatography, Affinity, Heterogeneous-Nuclear Ribonucleoproteins, Introns, Molecular Weight, Alternative Splicing, Gene Expression Regulation, Oligodeoxyribonucleotides, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, DNA Transposable Elements, Animals, Humans, Drosophila, Electrophoresis, Polyacrylamide Gel, HeLa Cells
Mammals, Base Sequence, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, Gene Expression, Exons, Chromatography, Affinity, Heterogeneous-Nuclear Ribonucleoproteins, Introns, Molecular Weight, Alternative Splicing, Gene Expression Regulation, Oligodeoxyribonucleotides, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, DNA Transposable Elements, Animals, Humans, Drosophila, Electrophoresis, Polyacrylamide Gel, HeLa Cells
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