Phosphatidylglucoside: a novel marker for adult neural stem cells
Copyright policy )Phosphatidylglucoside: a novel marker for adult neural stem cells
J. Neurochem. (2011) 116, 840–844.AbstractWe investigated the expression of a novel glycophospholipid, phosphatidylglucoside (PtdGlc), in adult mouse brains. Immunohistochemical analysis with DIM21 antibody, a monoclonal anti‐PtdGlc antibody, revealed robust PtdGlc staining in the two primary neurogenic regions of the adult rodent brain, the subventricular zone (SVZ) lining the lateral ventricle and the subgranular zone of the dentate gyrus. Intriguingly, the staining pattern of PtdGlc appeared to overlap that of glial fibrillary acidic protein, an adult neural stem cell marker in these regions. Further immunohistochemical analysis revealed that PtdGlc expression on the cell membranes of adult SVZ neural stem cells significantly overlapped with other proposed adult neural stem cell markers. Moreover, PtdGlc+ cells isolated from adult mouse SVZs by fluorescence‐activated cell sorting with anti‐PtdGlc antibody efficiently generated neurospheres in cell culture. These cells differentiated into neurons, astrocytes, and oligodendrocytes in vitro, directly demonstrating that PtdGlc‐expressing cells possessed multipotency. Our data suggest that PtdGlc could be a useful adult stem cell marker.
- RIKEN Brain Science Institute Japan
- RIKEN Japan
- Saitama University Japan
Neurons, Age Factors, Brain, Cell Differentiation, Glycerophospholipids, Embryo, Mammalian, Flow Cytometry, Mice, Inbred C57BL, Adult Stem Cells, Mice, Animals, Newborn, Bromodeoxyuridine, Tubulin, Glial Fibrillary Acidic Protein, Animals, 2',3'-Cyclic-Nucleotide Phosphodiesterases, Cells, Cultured
Neurons, Age Factors, Brain, Cell Differentiation, Glycerophospholipids, Embryo, Mammalian, Flow Cytometry, Mice, Inbred C57BL, Adult Stem Cells, Mice, Animals, Newborn, Bromodeoxyuridine, Tubulin, Glial Fibrillary Acidic Protein, Animals, 2',3'-Cyclic-Nucleotide Phosphodiesterases, Cells, Cultured
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