Representative example of cytofluorimetric analysis showing the effect of vitamin C alone or in combination with FAC, on the mitochondrial membrane potential of SH-SY5Y (panel A) and HTLA-230 (panel B) stained with JC-1 probe

Name: Representative example of cytofluorimetric analysis showing the effect of vitamin C alone or in combination with FAC, on the mitochondrial membrane potential of SH-SY5Y (panel A) and HTLA-230 (panel B) stained with JC-1 probe
Keywords: 3. Good health

Carosio, Roberta; Zuccari, Guendalina; Orienti, Isabella; Mangraviti, Salvatore; Montaldo, Paolo G

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Image . 2011

License: CC BY

Data sources: Datacite

figshare

Image . 2011

License: CC BY

Data sources: Datacite

Representative example of cytofluorimetric analysis showing the effect of vitamin C alone or in combination with FAC, on the mitochondrial membrane potential of SH-SY5Y (panel A) and HTLA-230 (panel B) stained with JC-1 probe

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2011Publisher:figshare

Authors: Carosio, Roberta; Zuccari, Guendalina; Orienti, Isabella; Mangraviti, Salvatore; Montaldo, Paolo G;

doi: 10.6084/m9.figshare.89446 , 10.6084/m9.figshare.89446.v1

Representative example of cytofluorimetric analysis showing the effect of vitamin C alone or in combination with FAC, on the mitochondrial membrane potential of SH-SY5Y (panel A) and HTLA-230 (panel B) stained with JC-1 probe

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Abstract

Copyright information:Taken from "Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake"Molecular Cancer 2007;6():55-55.Published online 30 Aug 2007PMCID:PMC2000471. In abscissa FL-1 (green fluorescence); in ordinate FL-2 (red fluorescence). Numbers represent the percentage of non-apoptotic red fluorescent cells (R1, R3) and that of apoptotic green fluorescent cells (R2, R4). Sodium ascorbate induces a marked increase in apoptosis which is fully prevented by treatment with FAC. SH-SY5Y and HTLA-230 were incubate in complete medium in the absence (A1, B1) or in presence of 1 mM sodium ascorbate (A2, B2) and 2 mM sodium ascorbate (A4, B4) for 16 hours. Cells were pretreated (A3, A5, B3, B5) with 70 μg/ml of FAC for 3 hours. Comparable results were obtained in three independent experiments.

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