miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis
Copyright policy )miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis
The piwi family genes are crucial for stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. However, their function in mammals remains unexplored. Here we report the cloning of a murine piwi gene (miwi) essential for spermatogenesis. miwi encodes a cytoplasmic protein specifically expressed in spermatocytes and spermatids. miwi(null) mice display spermatogenic arrest at the beginning of the round spermatid stage, resembling the phenotype of CREM, a master regulator of spermiogenesis. Furthermore, mRNAs of ACT (activator of CREM in testis) and CREM target genes are downregulated in miwi(null) testes. Whereas MIWI and CREM do not regulate each other's expression, MIWI complexes with mRNAs of ACT and CREM target genes. Hence, MIWI may control spermiogenesis by regulating the stability of these mRNAs.
- Duke University United States
- Duke University Hospital United States
- Duke University Health System United States
- Duke Medical Center United States
- Department of Cell Biology Duke University Medical Center United States
Male, Mice, Knockout, Cytoplasm, Molecular Sequence Data, Chromosome Mapping, Proteins, LIM Domain Proteins, Cyclic AMP Response Element Modulator, DNA-Binding Proteins, Repressor Proteins, Mice, Gene Expression Regulation, Argonaute Proteins, Animals, Protein Isoforms, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Spermatogenesis, Infertility, Male, Developmental Biology
Male, Mice, Knockout, Cytoplasm, Molecular Sequence Data, Chromosome Mapping, Proteins, LIM Domain Proteins, Cyclic AMP Response Element Modulator, DNA-Binding Proteins, Repressor Proteins, Mice, Gene Expression Regulation, Argonaute Proteins, Animals, Protein Isoforms, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Spermatogenesis, Infertility, Male, Developmental Biology
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