Interleukin‐3 or erythropoietin induced nuclear localization of protein kinase C β isoforms in hematopoietic target cells
Copyright policy )Interleukin‐3 or erythropoietin induced nuclear localization of protein kinase C β isoforms in hematopoietic target cells
AbstractProtein kinase C (PKC) has been implicated in the signal transduction pathways for the biological effect of both interleukin‐3 (IL‐3) and erythropoietin (EPO) in hematopoietic target cells. The goal of this study was to identify specific classical isoforms of PKC and their localization in hematopoietic cells in response to the growth factors, IL‐3 or EPO. In addition to murine fetal liver cells as a source of normal erythroid progenitor cells, we have utilized the B6SUt.EP cell line, a non‐transformed hematopoietic cell line that requires IL‐3 for proliferation, but for which EPO can substitute as a growth factor. With polyclonal antibodies prepared against peptide sequences specific for the α, βI, βII and γ isoforms of PKC, we have identified βI and βII as the predominant nuclear isoforms in target cells that proliferate in response to IL‐3 or EPO.
- Tulane University United States
Cell Nucleus, Immunoblotting, Cell Fractionation, Hematopoietic Stem Cells, Immunohistochemistry, Cell Compartmentation, Isoenzymes, Mice, Liver, Protein Kinase C beta, Image Processing, Computer-Assisted, Animals, Interleukin-3, Erythropoietin, Cells, Cultured, Protein Kinase C
Cell Nucleus, Immunoblotting, Cell Fractionation, Hematopoietic Stem Cells, Immunohistochemistry, Cell Compartmentation, Isoenzymes, Mice, Liver, Protein Kinase C beta, Image Processing, Computer-Assisted, Animals, Interleukin-3, Erythropoietin, Cells, Cultured, Protein Kinase C
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