Number of misassemblies for assembly results from different library sizes. Number of misassemblies for four different library sizes (300 bp, 400 bp, 500 bp and 600 bp) for E. coli DH1 and S. Parasanguinis FW213 are plotted against different depth of read coverage. A and B are from Group A [PE] which represents assembled results from all reads assembled as paired-end reads. C and D are from Group A [PE + SE] which represents assembled results from merged reads and all the non-overlapped paired end reads. E and F are from Group A [SE] which represents assembled results from all reads assembled as single-end reads. The number of misassemblies decreases as the depth of read coverage increases. Even though there are some fluctuations when depth of read coverage is low, the number of misassemblies reaches a steady number for all library sizes when depth of read coverage is high. (TIFF 882 kb)