Synthesis and Hybridization Properties of DNA–PNA Chimeras Carrying 5-Bromouracil and 5-Methylcytosine
Copyright policy )Synthesis and Hybridization Properties of DNA–PNA Chimeras Carrying 5-Bromouracil and 5-Methylcytosine
The preparation of 5-bromouracil and 5-methylcytosine peptide nucleic acid (PNA) monomers is described. These PNA monomers were used for the preparation of several DNA-PNA chimeras and their hybridization properties are described. The substitution of cytosine by 5-methylcytosine in DNA-PNA chimeras increased duplex stability while substitution of thymine by 5-bromouracil maintained it. Moreover, binding of DNA-PNA chimeras to double-stranded DNA to form triple helices was studied. In contrast to DNA, the presence of 5-methylcytosine and 5-bromouracil in DNA-PNA chimeras destabilized triple helix.
Peptide Nucleic Acids, Bromouracil, Cytosine, Magnetic Resonance Spectroscopy, Base Sequence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, 5-Methylcytosine, Nucleic Acid Hybridization, DNA
Peptide Nucleic Acids, Bromouracil, Cytosine, Magnetic Resonance Spectroscopy, Base Sequence, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, 5-Methylcytosine, Nucleic Acid Hybridization, DNA
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